INVESTIGADORES
GONZALEZ Paola Solange
artículos
Título:
Isolation and characterization of a Rhodococcus strain with phenol degrading ability and its potential use for tannery effluent biotreatment.
Autor/es:
CINTIA E. PAISIO; MELINA A. TALANO; PAOLA S. GONZÁLEZ; VICTOR D. BUSTO; JULIÁN RODRIGUEZ TALOU; ELIZABETH AGOSTINI
Revista:
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Editorial:
SPRINGER HEIDELBERG
Referencias:
Lugar: HEIDELBERG; Año: 2012 vol. 19 p. 3430 - 3439
ISSN:
0944-1344
Resumen:
Abstract
Introduction Wastewater derived from leather production
may contain phenols, which are highly toxic, and their
degradation could be possible through bioremediation
technologies.
Materials, methods and results In the present work, micro-
bial degradation of phenol was studied using a tolerant
bacterial strain, named CS1, isolated from tannery sedi-
ments. This strain was able to survive in the presence of
phenol at concentrations of up to 1,000 mg/L. On the
basis of morphological and biochemical properties, 16S
rRNA gene sequencing, and phylogenetic analysis, the
isolated strain was identified as Rhodococcus sp. Phenol
removal was evaluated at a lab-scale in Erlenmeyer
flasks and at a bioreactor scale in a stirred tank reactor.
Rhodococcus sp. CS1 was able to completely remove
phenol in a range of 200 to 1,000 mg/L in mineral
medium at 30 ± 2 °C and pH 7 as optimal conditions.
In the stirred tank bioreactor, we studied the effect of
some parameters, such as agitation (200?600 rpm) and
aeration (1?3 vvm), on growth and phenol removalefficiency. Faster phenol biodegradation was obtained in
the bioreactor than in Erlenmeyer flasks, and maximum
phenol removal was achieved at 400 rpm and 1 vvm in
only12h.Furthermore, Rhodococcus sp. CS1 strain was
able to grow and completely degrade phenols from tan-
nery effluents after 9 h of incubation.
Conclusion Based on these results, Rhodococcus sp. CS1
could be an appropriate microorganism for bioremediation
of tannery effluents or other phenol-containing wastewaters.