Novel tnpR-based transposable promoter traps suitable for RIVET studies in different Gram-negative bacteria

LOZANO M. J.; SALAS M. E.; GIUSTI M. A.; MARTINI M. C.; LÓPEZ J. L.; SALTO I. P.; DEL PAPA M. F.; PISTORIO M.; LAGARES A.

JOURNAL OF MICROBIOLOGICAL METHODS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2013 vol. 93 p. 9 - 11

0167-7012

The preparation of plasmid-borne RIVET libraries can be troublesome when high genomic coverages are needed. We present here the construction and functional validation of a new set of miniTn5 promoter traps to generate tnpR-based RIVET libraries. The ability to generate tnpR transcriptional fusions by transposition will significantly facilitate the setup of RIVET studies in those bacteria where Tn5 transposition is operative.