ALLLOPREGNANOLONE ALTERS THE LUTEINIZING HORMONE, PROLACTIN AND PROGESTERONE SERUM LEVELS INTERFERING WITH THE REGRESSION AND APOPTOSIS IN RAT CORPUS LUTEUM

LACONI MYRIAM; CAVICHIA JUAN CARLOS; FOSCOLO MABEL; SOSA ZULEMA; CHAVEZ CANDELARIA; YUNES ROBERTO; CABRERA RICARDO

HORMONE AND METABOLIC RESEARCH

GEORG THIEME VERLAG KG

Lugar: Dusseldorf; Año: 2012 vol. 44 p. 1 - 7

0018-5043

 Numero de Manuscrito: HMR 2011-09-0284 Adjunto nota acepatcion paper a continuacion del abstract Steroids synthesized in the central nervous system are termed “neurosteroids”. They are synthetisized and metabolized in several brain areas. The objectives of this work were: to determine if one i.c.v Allopregnanolone injection in rats could interfere in luteal regression and, to determine if Allopregnanolone could modify the serum LH, progesterone and prolactin release. Allopregnanolone was injected intracerebroventricular during proestrus afternoon and the animals were killed on oestrous morning. Ovulation test and histological analysis were performed in the oestrum morning with optic, electronic microscopy and TUNEL assay. The hormone assay was performed by radioinmunoassay. The Allopregnanolone injection significantly decreases luteinizing hormone serum level and the number of oocytes on oestrus. Progesterone and Prolactin serum levels were increased after Allopregnanolone injection. The inhibition of apoptotic figures was detected in the already formed corpora lutea belonging to the previous ovary cycle. When the GABAA antagonist, Bicucullin, was administered alone or with Allopregnanolone, any effect on the ovulation rate occurred and the apoptotic cell number were recovered, then this action was blocked by bicuculline (previously we shown the same profile when LH was analyzed). The results of this study established that centrally administered Allopregnanolone affects luteal regression through inhibition of apoptosis, and stimulation of progesterone production. The physiological significance of these findings is that Allopregnanolone promotes corpus luteum survival by multiple pro-survival signaling pathways comprising an integrate system. Dear Dr. Laconi,It is a pleasure to accept your manuscript entitled "Allopregnanolone alters the luteinizing hormone, prolactin and progesterone serum levels interfering with the regression and apoptosis in rat corpus luteum." in its re-revised form for publication in Hormone and Metabolic Research. You find your manuscript as well as this decision letter in your Author Center under Manuscripts with Decisions.Your manuscript will be forwarded to Georg Thieme Publishers. They will prepare your manuscript for printing. Thieme will contact you in the next weeks for further details.Thank you for your contribution. Also on behalf of the Editors of Hormone and Metabolic Research, we look forward to your continued cooperation to the journal.Yours sincerely,Werner A. Scherbaum, MD, PhDEditor-in-ChiefHormone and Metabolic ResearchE-mail: hmr@thieme.de