Interaction of the targeting sequence of chloroplast precursors with Hsp70 molecular chaperones

RIAL, D; ARAKAKI, A; CECCARELLI, EDUARDO AUGUSTO

EUROPEAN JOURNAL OF BIOCHEMISTRY

Federation of European Biochemical Societies

Año: 2000 vol. 267 p. 6239 - 6248

0014-2956

We have analyzed the interaction of DnaK and plant Hsp70 proteins with the wild-type ferredoxin-NADP1 reductase precursor (preFNR) and mutants containing amino-acid replacements in the targeting sequence. Using an algorithm already developed [RuÈdiger, S., Germeroth, L., Schneider-Mergener, J. & Bukau, B. (1997) EMBO J. 16, 1501±1507] we observed that 75% of the 727 plastid precursor proteins analyzed contained at leastone site with high likelihood of DnaK binding in their transit peptides. Statistical analysis showed a decrease of DnaK binding site frequency within the first 15 amino-acid residues of the transit peptides. Using fusion proteins we detected the interaction of DnaK with the transit peptide of the folded preFNR but not with the mature region of the protein. Discharge of DnaK from the presequence was favored by addition of MgATP. When a putative DnaK binding site was artificially added at the N-terminus of the mature protein, we observed formation of complexes with bacterial and plant Hsp70 molecular chaperones. Reducing the likelihood of DnaK binding by directed mutagenesis of the presequence increased the release of bound DnaK. The Hsp70 proteins from plastids and plant cell cytosol also interacted with the preFNR transit peptide. Overall results are discussed in the context of the proposed models to explain the organelle protein import.