INVESTIGADORES
ALLEVA Karina Edith
artículos
Título:
Cloning, functional characterization, and co-expression studies of a novel
Autor/es:
KARINA ALLEVA; MERCEDES MARQUEZ; NATALIA VILLAREAL; PAULA MUT; CLAUDIA BUSTAMANTE; JORGE BELLATI; GUSTAVO MARTINEZ; MARCOS CIVELLO; GABRIELA AMODEO
Revista:
JOURNAL OF EXPERIMENTAL BOTANY
Editorial:
OXFORD UNIV PRESS
Referencias:
Lugar: OXFORD; Año: 2010 vol. 61 p. 3935 - 3945
ISSN:
0022-0957
Resumen:
In strawberry, the putative participation of aquaporins should be considered
during fruit ripening. Furthermore, the availability of different firmness
cultivars in this non-climacteric fruit is a very useful tool to determine their
involvement in softening. In a previous work, the cloning of a strawberry
fruit-specific aquaporin, FaPIP1;1, which showed an expression profile associated
with fruit ripening was reported. Here, FaPIP2;1, an aquaporin subtype of PIP2
was cloned and its functional characterization in Xenopus oocytes determined. The
FaPIP2;1 gene encodes a water channel with high water permeability (P(f)) that is
regulated by cytosolic pH. Interestingly, the co-expression of both FaPIP
subtypes resulted in an enhancement of water permeability, showing P(f) values
that exceeds their individual contribution. The expression pattern of both
aquaporin subtypes in two cultivars with contrasting fruit firmness showed that
the firmer cultivar (Camarosa) has a higher accumulation of FaPIP1 and FaPIP2
mRNAs during fruit ripening when compared with the softer cultivar (Toyonoka). In
conclusion, not only FaPIP aquaporins showed an expression pattern associated
with fruit firmness but it was also shown that the enhancement of water transfer
through the plasma membrane is coupled to the presence/absence of the
co-expression of both subtypes.