INVESTIGADORES
GONZALEZ BARO Maria Del Rosario
artículos
Título:
Enzyme activities involved in lipid metabolism during embryonic development of Macrobrachium borellii
Autor/es:
GONZALEZ BARO MR; HERAS, H.; POLLERO, R.J.
Revista:
JOURNAL OF EXPERIMENTAL ZOOLOGY
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Año: 2000 vol. 286 p. 231 - 237
ISSN:
0022-104X
Resumen:
The activities of the enzymatic systems involved in the
activation and degradation of fatty acids, and in the synthesis of
triacylglycerols and phospholipids were studied in vitro using total cellular
homogenate and subcellular fractions of eggs of the shrimp Macrobrachium borellii
at different developing stages. Egg development was divided into seven stages
based on morphological features of the embryo. Palmitoyl-CoA ligase activity
increased as the embryo developed and showed its maximum at stage V. An
increase in the synthesis of triacylglycerols and diacylglycerols was also
observed at this stage. Diacylglycerylethers were synthesized more actively during
the first stages of development. The higher specific activity observed in total
homogenate than in microsomal fraction suggested that their synthesis was not
exclusively microsomal. Phospholipid synthesis was very active all along
development, reflecting active membrane biosynthesis. The highest activity of
the cytosolic triacylglycerol lipase was observed at stage V. Fatty acid
degradation, measured as mitochondrial b-oxidation activity, did not vary
significantly during development. We conclude that both the anabolic and
catabolic processes concerning lipid metabolism are very active, with values
similar to those described for adult hepatopancreas, revealing the major role
of lipids during shrimp embryogenesis energetics, and that the highest
activities of lipid synthesis-hydrolysis take place at stage V when embryos are
under active organogenesis.