PTHrP PROMOTES AN AGGRESSIVE BEHAVIOR OF CRC CELLS THROUGH ITS ACTION ON TUMOR AND STROMAL CELLS.

NOVOA DÍAZ MB; CARRIERE P; BIRKENSTOK C; PICARDI G; GOMEZ, L.; GIGOLA G; ZWENGER A; CALVO N; GENTILI C

Mar del Plata

Congreso; LXVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC).; 2023

Sociedad Argentina de Investigación Clínica (SAIC).

The interaction of several cells and molecules in the tumor microenvironment (TME) promotes tumor-aggressive features. Previously we found that parathyroid hormone-related peptide (PTHrP), a cytokine from TME, promotes an aggressive phenotype in HCT116 colorectal cancer (CRC) cells and that conditioned medium from PTHrP-treated HMEC-1 endothelial stromal cells (CM) induces epithelial to mesenchymal transition (EMT) in CRC cells. The aim of this work is to further investigate the PTHrP effects on CRC and its TME. HCT116 cells were treated with the CM. Western blot (WB) analysis showed that CM modulates the expression and activation of Met, a receptor related to CRC (p