STUDY OF PLACENTAL CELLS BEHAVIOR UNDER PTHrP EFFECT AND OTHER FACTORS OF THE CELLULAR ENVIRONMENT

NOVOA DÍAZ MB; CARRIERE P; BIRKENSTOK C; MACCHI R; CALVO N; SICA G; CANELLADA A; VINDEROLA G; GENTILI C

Mar del Plata

Congreso; LXVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC).; 2023

Sociedad Argentina de Investigación Clínica (SAIC).

Through paracrine signals, trophoblast cells invade the endometrium in early placentation. Thus, an unbalanced influence of environmental, maternal, and fetal factors on these cells can lead to gestational diseases. Parathyroid hormone-related peptide (PTHrP) expressed in the placenta may be one of these factors. It promotes epithelial-mesenchymal transition (EMT) and invasion, phenomena associated with physiological and pathological events. Other factors that could influence on placental structure and function are those derived from gut microbiota; in fact, Bifidobacterium is an attractive genus for its study in the gestational stage. This work aims to investigate if PTHrP influences trophoblastic cells phenotype and if so, whether human milk-derived Bifidobacterium animalis subsp. lactis INL1 (B. lactis INL1) is able to participate in its effect. We used HTR-8 and Bewo cells from normal placenta and choriocarcinoma, respectively, whose express PTHrP receptor, as revealed an in silico analysis. PTHrP at physiological concentration (10-12M) induces EMT-associated phenotype in HTR-8 cells (p