INVESTIGADORES
DALAMON Viviana Karina
artículos
Título:
Effects of pentanoate, 4 pentanoate, 3hidroxybutirate and insuline on the tissue-levels of long chain acylCoa and carnitine in the oxigenated and hypoxic rat atria
Autor/es:
VARELA A; DALAMON V; TESTONI G; CARREGAL M; CERRUTI S; SAVINO EA
Revista:
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
Editorial:
Taylor & Francis
Referencias:
Año: 1999 vol. 107 p. 22 - 26
ISSN:
1381-3455
Resumen:
Under hypoxic conditions the atrial contents of long-chain
acyl CoA (LCCoA) and long-chain acylcarnitine display a close
correlation with the noxious effects of fasting on the atrial functions
as well as with the amelioration effected by inhibitors of carnitine
palmitoyltransferase I. These findings suggested that fatty acid
oxidation was detrimental for the hypoxic atria. However, since changes
of the LCCoA and LCCa levels which may occur together with the hypoxic
disturbances attained under some other metabolic interventions had not
been assessed yet, present investigation aimed to provide information
about this issue. At the end of the prehypoxic equilibration period, all
the treatments tested evoked a fall of the free-CoA levels whereas
free-carnitine, LCCoA and LCCa remained unchanged. In the hypoxic atria,
4-pentenoate, an inhibitor of fatty acid β-oxidation that also can be
oxidized, did not change LCCoA and LCCa levels whereas the readily
oxidizable pentanoate evoked a drop of LCCoA. These effects may be due
to the trapping of CoA as the short-chain acyl esters of both
substances. Since 4-pentenoate and pentanoate were noxious on the
hypoxic atria even though they did not increase LCCoA and LCCa contents,
it may be inferred that short-chain acyl esters might be deleterious
during oxygen shortage. The exposure to 3-hydroxybutyrate, an oxidizable
substrate whose availability increases during fasting, did not alter
the LCCoA and LCCa contents, agreeing with the weak detrimental effects
that it exerts on the hypoxic atria. On the other hand, insulin elicited
a rise in the LCCoA and a fall in the LCCa contents. Inasmuch insulin
had been shown to improve the performance of the hypoxic atria, these
findings suggest that LCCoA might not be involved in the noxious effects
of fatty acid oxidation whereas LCCa would be the major toxic
catabolite.Read More: http://informahealthcare.com/doi/abs/10.1076/apab.107.1.22.4351