GFP- related Chromophores: Photoisomerization, Thermal Reversion, and DNA Labelling

FAILLACE, MARTIN SEBASTIÁN; DOLGOPOLOVA, EKATERINA A.; CEBALLOS, NOELIA MARCELA; RUIZ PEREYRA, ELBA NAHIR; LANFRI, LUCIA; ARGÜELLO, GUSTAVO ALEJANDRO; BURGOS PACI, MAXIMILIANO; SHUSTOVA, NATALIA; PELÁEZ, WALTER JOSÉ

PHYSICAL CHEMISTRY CHEMICAL PHYSICS

ROYAL SOC CHEMISTRY

Lugar: CAMBRIDGE; Año: 2023 p. 17943 - 17951

1463-9076

Due to pronounced effect of the confined environment on the photochemical properties of the 4-hydroxybenzylidine imidazolinone (HBI), a GFP-related chromophore, imidazolinone and imidazothiazolone analogues have been studied as fluorescent probes. Their photoisomerization and their thermal reversion were studied under 365-nm-irradiation, resulting in observance of an enthalpy-entropy compensation effect. Theoretical studies were carried out to shed light on the thermal reversion mechanism. Moreover, photophysical studies of benzylidene imidazothiazolone in the presence of dsDNA revealed fluorescence enhancement. The prepared compounds could be considered as a valuable tool for the detailed investigation of physicochemical, biochemical, or biological systems.THIOXOIMIDAZOLIDINONES IMIDAZOTHIAZOLONES PHOTOISOMERIZATION ENTHALPY-ENTROPY COMPENSATION EFFECT