Endometrial inflammatory cytokines: the effects of milk production levels and hormonal context on local expression of them during early lactation of dairy cattle.

PERALTA, M. B.; ANGELI, E.; STASSI, A.F.; CAINELLI, S.; DURANTE, L.; ORTEGA, H.H.; REY, F.; SALVETTI, N.R.; VELÁZQUEZ, MML.

Mar del Plata

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; 2018

Dairy cattle undergo metabolic and hormonal changes that impact in the immunological system during peripartum. Previous reports suggest that cows are immunosuppressed during postpartum and immune components of the reproductive tract are altered. We analyze the impact of milk production and the hormonal context on the expression of endometrial cytokines. The animals were divided in high production group (HP: > 4800 L, n = 11) and low production group (LP: < 4400 L, n = 13) considering the average of the dairy production up to 150 days in lactation. Blood samples and endometrial biopsies were obtained at 45 and 60 days postpartum (dpp). The mRNA expression of interleukin IL1α, TNFα and IL6 was evaluated by real time PCR. The protein expression was analyzed by immunohistochemistry in the endometrial luminal epithelium (LE), glandular epithelium (GE) and stroma (S). Plasmatic concentrations of cortisol, estrogen and progesterone were measured by ECLIA. A general linear model was used to analyze the results. The results showed that mRNA levels of IL1α were lower at 60 dpp than 45 dpp, and LP group showed a decrease of IL1α mRNA expression in relation to HP group (p