INVESTIGADORES
GARRIDO Mariano Enrique
artículos
Título:
Green method based on a flow-batch analyzer system for the simultaneous determination of ciprofloxacin and dexamethasone in pharmaceuticals using a chemometric approach
Autor/es:
M. F. RAZUC; M. GRÜNHUT; E. SAIDMAN; M. GARRIDO; B. S. FERNÁNDEZ BAND
Revista:
TALANTA
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013
ISSN:
0039-9140
Resumen:
A green FBA method with UV detection was developed for simultaneous
determination of ciprofloxacin (CIP) and dexamethasone (DEX) in ophthalmic and
otic preparations. A lab-made mixing detection chamber (MDC) was designed and
coupled to the spectrophotometer in order to perform the mixing of solutions and
the detection in the same receptacle. Only water was used as solvent and no
previous separation of the components was required. Both analytes have a strong
absorption between 190 and 370 nm in aqueous medium, at pH 7. However, the
spectrum of DEX is embedded in the CIP spectrum. Thus, while CIP was analyzed
using univariate calibration, DEX analysis was carried out comparing partial least
squares (PLS-1) and multiple linear regression (MLR). The latest required a
previous variable selection step, which was performed using the genetic algorithm
(GA) and the successive projections algorithm (SPA). The FBA system made it
possible to automatically prepare the calibration and validation sets. The statistical
parameters, in terms of relative errors of calibration and prediction, were
acceptable for the determination of both CIP and DEX. Also, a comparative study
of chemometric models was carried out. Commercial samples were analyzed and
the obtained results are in close agreement with HPLC pharmacopeia methods.
The joint interval test for the slope and the intercept was used to test for the
presence of bias. There were no statistical differences between the proposed
method and the reference method ( = 0.05). The sample throughput was 10 h-1.
The combination of automation and chemometric tools allows us to develop an
environmental friendly method for the quality control of CIP and DEX in
pharmaceuticals.
possible to automatically prepare the calibration and validation sets. The statistical
parameters, in terms of relative errors of calibration and prediction, were
acceptable for the determination of both CIP and DEX. Also, a comparative study
of chemometric models was carried out. Commercial samples were analyzed and
the obtained results are in close agreement with HPLC pharmacopeia methods.
The joint interval test for the slope and the intercept was used to test for the
presence of bias. There were no statistical differences between the proposed
method and the reference method ( = 0.05). The sample throughput was 10 h-1.
The combination of automation and chemometric tools allows us to develop an
environmental friendly method for the quality control of CIP and DEX in
pharmaceuticals.
The combination of automation and chemometric tools allows us to develop an
environmental friendly method for the quality control of CIP and DEX in
pharmaceuticals.