Analysis of Transcriptional Feedback Strategy for Reducing Interaction in Gene Expression Processes

SEBASTIÁN NUÑEZ; FABRICIO GARELLI; JESÚS PICO; HERNÁN DE BATTISTA

Florianópolis

Simposio; 12th IFAC Symposium on Dynamics and Control of Process Systems, including Biosystems (DYCOPS 2019); 2019

IFAC

Advances in genetic manipulation have allowed the overexpression of proteins andinsertion of circuits in cells. However, the expected behaviour can be altered by the internalcompetition for the limited amount of cellular resources. In this work we analyse a feedbackstrategy based on transcription inhibition that aims to reduce the interaction in a two-proteinexpression system. The results allow interpreting the effects of negative feedback on the steadystateprotein levels and how the realizable protein region is affected by the feedback loop.