Eliminación renal de aniones orgánicos en ratas con insuficiencia renal crónica

TORRES, ADRIANA; BRANDONI, ANABEL; VILLAR, SILVINA; OTTAVIANO, GRACIELA; MULLER, ANGÉLICA; PICENA, J. CARLOS; MARTINEZ, ALEJANDRA; MAC LAUGHLIN, MYRIAM

Rosario

Congreso; XXIII Reunion Anual de la Sociedad de Biología; 2003

Sociedad de Biología (SBR)

We have observed a reduction in the systemic depuration of p-aminohippuric (PAH) acid, in rats with chronic renal failure (CRF). In this work we evaluated renal excretion of PAH in malee Wistar rats wih CRF (n = 6). A parallel control (C) group (n=4) was also processed. We measured systolic blood pressure (SBP) plasma creatinine(Cr, mg%) and excreted, secreted and filtered PAH loads ( EL, SL, FL , m g/min/100 body weight) At the end of the experiments , the remnant kidney masses were removed and processed for histological studies, and to evaluate the amount of the organic anion transport protein (OAT1) present in the basolateral membrane of proximal tubular cells (S2 segment) by inmunohistochemestry. The statistical analysis was done using the t-test for unpaired data (*p<0.05). The results were collected in the table below: The histological studies showed that kidneys of the CRF group had lymphocitic infiltrate in the interstitium, a moderate dilation of proximal tubules with decreased cell height and tubular tiroidization. The CRF group also had a reduced amount of OAT1, as detected by inmunohistological studies. Thus, there was a correlation between functional and morphological studies. We conclude that, in CRF, there is a decrease in the renal excretion of PAH load which could be explained by either decreased PAH filtration load, or by a reduction in PAH secreted load, which, in turn, could be attributed, at least in part, by an altered expression of the OAT1 protein. The decrease in the excreted PAH load could be explained by a reduction in the systemic PAH depuration.