Eliminación renal de aniones orgánicos a las 48 h postliberación de la obstrucción de ambos uréteres en ratas

VILLAR, SILVINA RAQUEL; BRANDONI ANABEL; TORRES, ADRIANA MONICA

Rosario

Congreso; XXIII Reunión Anual de la Sociedad de Biología de Rosario.; 2003

Sociedad de Biología de Rosario.

The obstructive uropathy is a disease produced by obstruction of the urinary tract. One of the proteins that allows renal detoxification of organic anions from the body is the Organic Anion Transporter 1 (OAT1). The aim of this study was to evaluate the renal capability to excrete organic anions in rats with bilateral ureteral obstruction (BUO). Adult male Wistar rats were employed. Both ureters were ligated during 24 h, then they were released and the studies were done after 48 h (BUO, n=6). A parallel group of sham rats (Sham, n=5) was employed. Plasma urea leves were evaluated by spectrofhotometric technique (Up). P-aminohippurate (PAH) renal clearance (ClPAH), excreted (ELPAH), filtrated (FLPAH) and secreted (SLPAH) loads were determined employing conventional clearance techniques. The abundance of the renal OAT1 was determined by Electrophoresis and Western Blot.(*p< 0.05). Results:   Sham OUB Up (g/L) 0.62 ± 0.07 1.78 ± 0.25* ClPAH (mL/min/100g b.w.) 4.12 ± 0.09 0.83 ± 0.15* ELPAH (m g/min/100g b.w.) 165.3 ± 6.3 89.7 ± 12.4* FLPAH (m g/min/100g b.w.) 22.9 ± 1.1 20.3 ± 3.6 SLPAH (m g/min/100g b.w.) 134.2 ± 8.8 69.3 ± 10.2* OAT1 (%) 100 ± 6 44 ± 4* In this experimental model of BUO a diminution in the ClPAH was observed after 48 h release of the obstruction. The lower ELPAH was determined by the lower SLPAH. This could be explained, at least to some extent, for the diminution in the abundance of the OAT1.