OAT1 AND OAT3 EXPRESSION IN ACUTE RENAL FAILURE (ARF).

DI GIUSTO, GISELA; VILLAR, SILVINA RAQUEL; TORRES, ADRIANA MÓNICA

Tandil, Buenos Aires.

Congreso; 40 Reunión anual de SAFE.; 2008

SAFE, Sociedad Argentina de Farmacología Experimental

OAT1 AND OAT3 EXPRESSION IN ACUTE RENAL FAILURE (ARF). Di Giusto G., Villar S.R., Torres A.M. Area Farmacología. Facultad de Ciencias. Bioquímicas y Farmacéuticas. UNR. CONICET. E-mail: giseladg@hotmail.com.   The renal expression of the organic anion transporters (Oat1 and Oat3) was evaluated in three experimental models of ARF using Male Wistar rats. Ischemic ARF (I/R, n=4) was induced by occlusion of both renal pedicles during 60 min, followed by 60 min of reperfusion. Nephrotoxic ARF (HgCl2, n=4) was produced by administering a single dose of HgCl2 (5 mg/kg b.wt., s.c.) 18 h before. Post-renal ARF (BUO, n=4) was obtained by bilateral obstruction of ureters for 24 h plus a releasing period of 24 h. A parallel group of sham operated rats was employed. Oat1 and Oat3 expression was evaluated by immunohistochemistry and by immunoblotting (homogenates (H) and basolateral membranes (M)). Global renal function was assessed by urea plasma levels. (*) P < 0.05. Sham I/R HgCl2 BUO Uremia (g/L) 0.46 ± 0.02 0.58 ± 0.01* 1.25 ± 0.09* 2.91 ± 0.3* Oat1 H (%) 100 ± 5 70 ± 6* 145 ± 8* 156 ± 8* Oat1 M (%) 100 ± 2 87 ± 3* 70 ± 4* 24 ± 9* Oat3 H (%) 100 ± 5 78 ± 5* 68 ± 7* 48 ± 11* Oat3 M (%) 100 ± 3 81 ± 6* 76 ± 4* 35 ± 3* Immunohistochemistry confirmed these results. A good correlation was observed between uremia and Oat1 M (r = 0.99) and Oat3 M (r = 0.97). These data suggest that nitrogenous waste products might be involved in the regulation of both carriers expression.