Expresión de AQP2 en respuesta a Obstrucción Ureteral Bilateral.

VILLAR, SILVINA RAQUEL; TORRES, ADRIANA MÓNICA

Tandil Buenos Aires

Congreso; 40 Reunión anual-SAFE; 2008

Sociedad Argentina de Farmacología Experimental (SAFE)

AQP2 EXPRESSION IN RESPONSE TO BILATERAL URETERAL OBSTRUCTION. Villar S.R., Torres A.M. Area Farmacología. Facultad de Cs. Bioquímicas y Farmacéuticas. UNR. CONICET. E-mail: villar_silvina@hotmail.com. It was demonstrated that bilateral ureteral obstruction (B) is associated with postobstructive diuresis 1 day after obstruction releasing, being normalized after 7 days. The aim of this work was to study the time course effect of B on aquaporin 2 (AQP2) protein abundance (Prot.) in apical membranes and mRNA in rat kidney. Ureters were obstructed for 24 h in all experiments, and released for 1 (B1, n=4), 2 (B2, n=4) and 7 (B7, n=4) days. A parallel group of Sham rats (S, n=4) was employed. AQP2 (%) expression was determined by Immunoblotting and RT-PCR techniques in renal cortex (Cx) and medulla (Med). Data were analysed with ANOVA plus Newman-Keuls P<0.05: [a]vsS, [b]vsB1, [c]vsB2, [d]vsB7. AQP2 Prot. (Cx) Prot. (Med.) mRNA (Cx) mRNA (Med) S 100±5 100±2 100±5 100±6 B1 71±7 a,c,d 72±6 a,c,d 60±3 a,c,d 49±3 a B2 129±8 a,b 120 ± 14 b,d 84±8 b,d 56±2 a B7 149±7 a,b 152 ± 10 a,b,c 154±4 a,b,c 57±2 a The decreased expression of AQP2 in B1, might contribute to the obstructive polyuria. In B2 and B7 groups, the increased AQP2 expression in apical membranes might be sufficient to normalize water reabsorption. The no parallel changes in protein and mRNA levels suggest that the cellular responses to obstruction might involve different regulatory processes.