ENFERMEDAD DE CHAGAS: ANÁLISIS DE MARCADORES DE SENSCENCIA DE GLOBULOS ROJOS

ROCIO STAMPONE; ANTONELLA PACINI; ALEJANDRA ENSINCK; BRENDA DINATALE, ; ANA ROSA PÉREZ; CARLOS COTORRUELO; SILVINA VILLAR

Rosario

Congreso; XIV Congreso y XLII Reunión Anual de la Sociedad de Biología de Rosario; 2022

RED BLOOD CELL SENESCENCE IN CHAGAS DISEASERocío Stampone1, Antonela Pacini1, Alejandra Ensinck2, Brenda Dinatale1, Ana Rosa Pérez1, Carlos Cotorruelo1, Silvina Villar11Instituto de Inmunología Clínica y Experimental Rosario (IDICER-CONICET-UNR), Rosario, Argentina2Cátedra de Inmunología. Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Rosario, ArgentinaE-mail: rociostampone@gmail.com It is known that red blood cells (RBC) have a life expectancy of 120 days; however, there is evidence that supports RBC ability to modify its life expectancy due to chronic inflammatory processes. Chagas disease (CD) is an infectious and inflammatory process with the possibility of modifying the half-life of the RBC. This study aims to identify possible aging mechanisms of the red cells in patients with CD. For this, the relationship between the aging of the red cells and the changes in its membranes which lead to clearance of senescent cells was investigated. Our study was designed to evaluate young red cells (YRC) and senescent red cells (SRC) in individuals without chronic Chagas cardiomyopathy (Indeterminate form IND, n=4) and with chronic Chagas cardiomyopathy (CCC, n=4). Healthy volunteers were matched according to sex and age (Co, n=5). Flow cytometry has been applied for detection of RBC bound Immunoglobulin G (IgG), CD47 and Annexin V. *p