Collection and documentation of isolates of the fungus Phaeoisariopsis griseola in Argentina.

STENGLEIN, S.A.; FERMOSELLE, G.E.; VIZGARRA, O.N.; PLOPER, L.D.; BALATTI, P.A.

Montevideo

Simposio; V Simposio de Recursos Genéticos para la América Latina y el Caribe.; 2005

Phaeoisariopsis griseola (Sacc.) Ferraris is the causative agent of a bean (Phaseolus vulgaris L.) disease known as Angular Leaf Spot. We want to develop strategies to control the disease therefore it is crucial to know in advance the variability of the pathogen and its genetics. Because of this, the purpose of this work was to isolate the causative agent of ALS from many different areas cultivated with different bean germplasm, which were sampled at random. We isolated 67 representatives of the fungus from the following locations El Quemado and Palpalá (province of Jujuy); Rrio. de Lerma, Cerrillos, La Merced, Metán, Chicoana and La Silleta (Salta); Zárate, Trancas and Vipos (Tucumán); Pozo Hondo (Sgo. del Estero). Bean germplasms sampled were among others TUC 390, TUC 500, TUC 510, Camilo INTA, NAG 12, Overito and Alubia Cerrillos. In addition to this, isolates from Phaseolus coccineus were also collected from Tafí del Valle, Tucumán. This collection is kept in sterile filter paper at -20°C at the Instituto de Fisiología Vegetal, UNLP. We are currently analyzing variability of the fungus by means of a set of bean differentials and genetic diversity by molecular markers such as RAPD and ISSR.