Effect of high-pressure treatment on structural properties of low density lipoprotein of hen yolk

PUPPO, M.C*; CHAPLEAU, N.; SPERONI, F.; DE LAMBELLERIE-ANTON, M.; AÑÓN, M.C.; AND ANTON, M.

Montepellier, Francia

Conferencia; International Conference Engineering and Food - ICEF9; 2004

Changes in LDL structure of acidic (LDL3) and alkaline (LDL8) dispersions (1% w/v) due to HP treatment (200-600 MPa) was studied. Protein structure changes and aggregation phenomenon were analyzed by protein solubility, surface hydrophobicity, free sulfhydryls, circular dichroism, size exclusion chromatography, and quasi-elastic light scattering and native electrophoresis. Secondary structure changes followed by aggregation were observed for LDL8 at 600 MPa. This behavior was accompanied with a decrease in H0 and an increase in SHF. No effect on LDL3 structure with HP was observed. in SHF. No effect on LDL3 structure with HP was observed. in SHF. No effect on LDL3 structure with HP was observed. 0 and an increase in SHF. No effect on LDL3 structure with HP was observed.F. No effect on LDL3 structure with HP was observed.