Anti-ManLAM antibodies circulating in Multi Drug Resistant (MDR) and Drug-sensitive tuberculosis (TB) patients exert differential effect on fagocytosis and costimulatory potential of Dendritic Cells

SCHIERLOH P; COLMAN E; LANDONI V; LABORDE E; BASILE J; BALBOA L; SABIO Y GARCÍA C; ROMERO MM; GEFFNER L; BARRERA L; CASTAGNINO J; SASIAIN MC

Buenos Aires

Congreso; 1er Congreso Franco Argentino de Inmunología; 2010

Mannosilated lipoarabinomannan (ManLAM) is one of the most abundant glycolipid in cell wall of slow-growers pathogenic mycobacteria. Its immunomodulatory action in tuberculosis (TB) disease has been frequently associated with recognition by innate immune receptors displayed by host cells. Circulating anti-ManLAM antibodies (αManLAM Abs) has been detected in TB patients but its role in physiopathology remains poorly studied. In present communication, we asked if αManLAM Abs may affect dendritic cell (DC) function and factors/mechanisms that are involved. To this end, we characterize quantity and quality of αManLAM Abs present in serum samples of Multi Drug Resistant (MDR; N=20) and Drug-sensitive TB patients (DS; N=20) using non-TB donors as control population (N=17). Next, we selected a well characterized subgroup of sera (MDR, N=6; DS, N=6) to perform bioassays employing normal blood monocytes derived DC. Immunochemical characterization of sera by ELISA, Competition ELISA and Bacterial based ELISA techniques shows heterogeneous αManLAM response in terms of serum titers, chemical nature of epitope and mycobacterial wall surface epitope-display, with no significant differences among MDR and DS patients but both above control group (p