REWIRING OF MACROPHAGE METABOLISM BY LIPID MEDIATORS DERIVED FROM OMEGA-3 FATTY ACIDS PRESENT IN TUBERCULOUS PLEURAL EFFUSIONS: ITS IMPACT ON THE CONTROL OF MYCOBACTERIUM TUBERCULOSIS INFECTION

JOAQUINA BARROS; MARIANO MAIO; JOSÉ LUIS MARÍN FRANCO; MARINE JOLY; DOMINGO PALMERO; XAVIER ARAGONE; RAFAEL J ARGÜELLO; EMILIE LAYRE; GEANNCARLO LUGO-VILLARINO; OLIVIER NEYROLLES; CHRISTEL VÉROLLET; MARÍA DEL CARMEN SASIAIN; LUCIANA BALBOA

Congreso; LXX Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2022

The success of M. tuberculosis (Mtb) as a pathogen derives from its efficient adaptation to the intracellular milieu of macrophages, entailing the regulation of their metabolic pathways. Previously, we found that M1 macrophages exposed to the acellular fraction of pleural effusions from TB patients (TB-PE) displayed a reduced glycolytic activity and an increased mitochondrial respiration by targeting HIF-1α expression, and ultimately impairing the resistance to infection. Such properties were driven by polyunsaturated fatty acids (PUFA) metabolites, and herein, we aim to identify them. For this purpose, were determined PUFA metabolites within TB-PE by LC-MS/MS. The abundances of 18-HEPE, 7(R)-Maresin 1, Protectin Dx and Resolvin D5 correlated with the inhibition of M1 macrophages’ glycolysis (p