ANTIGENOTOXIC ACTION OF Minthostachys verticillata ESSENTIAL OIL AGAINST DAMAGE INDUCED BY AFLATOXIN B1 IN RATS

ESCOBAR F.M.; SABINI C.; CARIDDI L.; TORRES C.; PEREYRA C.; MAGNOLI A.; CAVAGLIERI L.R.

Tafí del Valle, Túcuman

Jornada; XXXIII ANNUAL SCIENTIFIC MEETING TUCUMAN BIOLOGY ASSOCIATION; 2017

Aflatoxin B1 (AFB1) is the most toxic mycotoxin and it is a common contaminant in foods. M. verticillata (peperina) is a plant of great ethnobotanical interest with multiple demonstrated bioactivities. The objective of this work was to determine the antigenotoxic activity of M. verticillata essential oil (EO) against injury induced by AFB1 in rat bone marrow. Wistar rats were divided into four groups with 5 rats per group: group 1 common food (negative control); group 2 common food + EO (EO control); group 3 food with AFB1 [4 μg/kg] (toxin control); group 4 food with AFB1 + EO. The EO was dissolved in water at 0.04% (phospholipids emulsifying agents) to be administered in the drinking water. Food and water were available ad libitum throughout the experimental period (45 days). At the end of the study, the rats were decapitated and the femurs were immediately excised from the body. For each animal 2000 erythrocytes were counted to determine the frequency of micronuclei (MN). The average micronucleated erythrocytes (MNE) was 2.66? for the negative control group and 2.41? for the group treated with EO at 0.04%. In contrast, rats treated with AFB1 showed a significant increase in MNE compared to other groups, with mean values of 4.67?*. The antigenotoxic effect of EO was observed in group 4 (AE + AFB1). In this group, a decrease in the number of MN was observed compared to the group treated only with the toxin. A similar average value of MNE compared to the negative control group was observed (3.01?).These results suggest that M.verticillataEO exerts an antigenotoxic action against damage induced by AFB1 in hematopoietic bone marrow cells of rat.