IN VITRO CITOTOXICITY OF ACACETIN, A FLAVONOID IDENTIFIED IN AQUEOUS EXTRACT OF Baccharis articulata

ESCOBAR F.M.; ARGUELLO F.C.; TORRES C.

Tafí del Valle, Túcuman

Jornada; XXXIII ANNUAL SCIENTIFIC MEETING TUCUMAN BIOLOGY ASSOCIATION; 2017

Secondary metabolites, of phenolic nature, from different species of vegetables are responsible for various pharmacological properties used in traditional or complementary medicine. Baccharis articulate is a common plant in Córdoba hills and is often used in traditional medicine because of its hepatoprotective and cholagogue properties. In previous works we showed the antiviral action of the hot aqueous extract of B. articulata at non cytogenotoxic concentrations. Acacetin is relevant among the flavonoids found in this extract. Flavonoids are substances with multiple positive effects on human and animal health but information about their general toxicity is scarce. So the objective of this work was to determine the cytotoxic action of acacetin in the Vero cell line. Cytotoxic Concentration 50% (CC50) was determined by tetrazolium salt reduction (MTT) and Maximum Non Cytotoxic Concentration (MNCC). Assayed concentrations varied from 1 to 500 μg/ml. Acacetin altered cells morphology at most concentrations tested, producing monolayer retraction and cell lysis. The NCMC was 15 μg/ml and CC50 was 8.42 μg/ml. The present work provides preliminary results on the toxicity of acacetin.