ANTIOXIDANT COMPOUNDS OF Achyrocline satureioides INHIBIT HERPES SUIS TYPE 1 VIRUS

MENIS CANDELA F; SABINI C.; ESCOBAR F.M.; TORRES C.; CARIDDI N.; COMINI L.; SABINI L.

Tafí del Valle, Túcuman

Jornada; XXXIII ANNUAL SCIENTIFIC MEETING TUCUMAN BIOLOGY ASSOCIATION; 2017

Herpes viruses represent a high impact health problem. Herpes suis type 1 virus causes Aujeszky's disease and results in significant economic losses to swine breeders. Medicinal plants can be useful to solve this problem. Achyrocline satureioides,"marcela del campo", has antimicrobial, immunomodulatory and antiviral properties. Phytochemical analysis of A. satureioides cold aqueous extract indicated the presence of luteolin (L), quercetin (Q), chlorogenic acid (CHLA) and caffeic acid (CA). The purpose of this study was to evaluate the in vitro antiviral action of the compounds (C) of A. satureioides against the Herpes suis type 1 virus. Cytotoxicity was assessed on Vero cells by Uptake of Neutral Red (UNR) and MTT reduction assays. Then, the antiviral activity of C throughout the viral replication cycle was evaluated by plaque reduction assay. Further, the action stage during the replication cycle was determined. Finally, the selectivity index (SI) was calculated. Cytotoxicity studies revealed low toxicity of all C. Antiviral ability studies indicated that H. suis type 1 virus was inhibited in 66% by L at 15 μg/ml. In contrast, CA, Q and CHLA were not active against the virus. The action stage study indicated that L exerts its action in the post-viral adsorption and penetration stage. Selectivity indices of L were 105.3 (NRU) and 72.6 (MTT). In conclusion, L showed strong selective inhibitory ability against H. suis type 1 virus and it may be useful for herpes virus infections treatment.