Selective action of decoction obtained from Minthostachys verticillata against fungi and bacteria by in vitro cytogenotoxical and antimicrobial assays

SABINI L.; ESCOBAR F.; TORRES C.; CASASNOVA F.; DEMO M.; CORDERO GABRIELLI P.; ZANON S.; SUTIL S.

Tafi del Valle, Tucumán

Jornada; XXIII Jornadas Científicas Organizadas por La Asociación de Biología de Tucumán; 2006

The capacity of the decoction (D) obtained from M. verticillata was evaluated in its antimicrobial activity and the selective action. The initial concentration of D was 34.59 mg/ml. The MNCC value, determined over Vero cells in monolayer was of 4.32 mg/ml. The cytotoxic alterations, evaluated by the Allium cepa L. test, demonstrated that there were not statistically significant differences in the modification of the mitotic index and in the physiological and clastogenics aberrations between treatments vs control. Data indicated absence of genotoxicity. The MICs values were of: 0,144 mg/ml for H. pylori and S. aureus, determined by disk diffusion methods and broth microdilution test (BMT), respectively. Whereas the MIC for Rhodotorula spp. was 0.033 mg/ml, and 0.067 mg/ml for C. neoformans, also defined by BMT. Microorganisms C. albicans, E. coli, S. epidermidis, B. cereus, P. aeruginosa and Klebsiella sp were not inhibited in their development by decoction. The chemotherapeutical index was in ranks of 30 to 130. At the active concentrations against to sensitive microorganisms the absence of cytotoxic and genotoxic damages reveals a strong antimicrobial selective action to control certain pathologies by means of the use of M. verticillataLs decoction with a great security.