Evaluation of the South American gastropod Heleobia parchappii as test organism in cadmium toxicity bioassays

ACHIORNO CECILIA; MINARDI GRACIELA; SCHNEIDER, MARCELA INES; FOGEL, MARILINA NOELIA

ECOLOGÍA AUSTRAL

ASOCIACIÓN ARGENTINA DE ECOLOGÍA

Año: 2023

0327-5477

Gastropods are abundant key components in freshwater ecosystems, many of which are affectedby anthropogenic activities. The genus Heleobia is widely distributed across Argentina, and the native Heleobiaparchappii is very common in the Pampean Region. The identification of native candidate species for toxicitytesting is gaining increasing interest. The objectives of the present study were to evaluate 1) the susceptibilityof H. parchappii to a reference toxicant (Cl2Cd), and 2) its suitability for toxicity testing by estimating LC50.Also, we discussed the applicability of the proposed protocol. Snail sensitivity was assessed by exposure toconcentrations between 0.5 and 12 mg Cd/L under acute static conditions during 72 h (exposure period). Then,snails were transferred to the control medium for 24 h (post-exposure period) and checked for recovery andthose not responding to stimulation were considered to be dead. The endpoints were snail immobilization(recorded every 24 h during the exposure period) and mortality at the end of the post-exposure period. Snailsensitivity was analyzed with a generalized linear mixed model (GLMM) and LC50 (lethal concentration 50%)was calculated using probit analysis. Results indicated that the number of immobilized snails increased withincreasing concentration. The toxic effect of Cd on snails persisted 24 h after exposure. The LC50 was 2.145(1.675-2.641) mg Cd/L. The sensitivity of H. parchappii was similar to that reported for other freshwater standardspecies, supporting its suitability as test organism. The protocol was appropriate for use in toxicity testing asit is simple, inexpensive and reproducible.