IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model

GÁLVEZ, M.; GIORGI, G.; LENCINAS, I.; CRESCITELLI, M. C. ; KENNY, A.; MARTÍNEZ, A.; MURRAY, M. G.; PRAT, M. I.

Congreso; LXVII Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC). LXX Reunión anual de la Sociedad Argentina de Inmunología (SAI) 3er Congreso Franco-Argentino de Inmunología (FAIC). Reunión anual 2022 de la Sociedad Argentina de Fisiología; 2022

Allergic rhinitis (AR) has worldwide distribution with an increasingprevalence every year. Also, allergic rhinitis and chronic rhinosinusitisare frequently associated with asthma. In our region, Russianthistle (Salsola kali) is a common weed causing pollinosis. The aimof this study was to establish a mouse model of AR based on Salsolakali pollen proteins, and to define the extent that this could have inthe lower airways. BALB/c mice (n = 5/group) were administratedweekly with PBS or S. kali pollen extract through i.p. route and laterchallenged by nasal instillation of PBS or S. kali pollen respectivelyon days 21, 22 and 23. Bronchoalveolar lavage (BAL) wereobtained. Serum total IgE and S. kali-specific IgE were measuredby ELISA. After sacrifice, the noses and lungs were fixed and paraffinembedded for histological analysis (H&E, toluidine blue and periodic acid-Schiff). After nasal challenge, frequency of sneezingin sensitized group were higher than the control (p