Analysis of Pseudomonas community structure in soil and rhizosphere of agricultural plots under no-till management in Argentina

AGARAS, B; WALL LG; VALVERDE C

Congreso; PSEUDOMONAS 2013 CONFERENCE; 2013

In Argentina, 78.5% of agricultural soils are cultivated under no-till management. The consortium BIOSPAS was created to evaluate the effects of this practice on the biology and productivity of soils. One of the focus groups is the pseudomonads, widely recognized as PGPR. We sampled soil and rhizosphere from plots under good (GAP) or bad agricultural practices (BAP), as defined by farmers. Neighboring grasslands with no cropping history (NE) served as reference. Samples were collected in February and September during 2010 and 2011. Viable pseudomonads were counted on Gould?s S1 selective plates (TP) and fluorescent pseudomonads (FP) were enumerated under UV light. Those plates bearing the most abundant pseudomonads from each sample, served as DNA source to carry out PCR-RFLP community structure analysis of the Pseudomonas marker genes oprF and gacA.. Fingerprints were analyzed using Gelcompar II v.4.602? (SIGUE)