Influence of chitosan, acetic acid and citric acid on growth and tropane alkaloid production in transformed roots of Brugmansia candida Effect of medium pH and growth phase

PITTA-ALVAREZ, S.I.; GIUILIETTI, ANA MARÍA

PLANT CELL TISSUE AND ORGAN CULTURE

SPRINGER

Lugar: Berlin; Año: 2000 vol. 59 p. 31 - 38

0167-6857

The effects of chitosan, acetic acid and citric acid on production and release of hyoscyamine and scopolaminein hairy root cultures of Brugmansia candida were studied. Chitosan and acetic acid were tested at differentconcentrations and also at different media pH values. At pH 5.5, and at certain concentrations, acetic acid andchitosan increased the content of root scopolamine and hyoscyamine, and promoted the release of both alkaloids.Lowering the pH to 3.5 and 4.5 reduced the accumulation of both alkaloids in the roots, but at a pH of 4.5, theirrelease increased significantly. Acetic and citric acid stimulated the release of scopolamine and hyoscyamine.