INVESTIGADORES
LIBKIND FRATI Diego
artículos
Título:
The genome sequence of Saccharomyces eubayanus and the domestication of lager-brewing yeasts
Autor/es:
BAKER, E.; WANG, B.; BELLORA, N; PERIS, D.; HULFACHOR, A.; KOSHALEK, A. ; LIBKIND, DIEGO; HITTINGER, C.
Revista:
MOLECULAR BIOLOGY AND EVOLUTION
Editorial:
OXFORD UNIV PRESS
Referencias:
Lugar: Oxford; Año: 2015 vol. 32 p. 2818 - 2831
ISSN:
0737-4038
Resumen:
The dramatic phenotypic changes that occur in organisms during domestication leave indelible imprints on theirgenomes. Although many domesticated plants and animals have been systematically compared with their wild geneticstocks, the molecular and genomic processes underlying fungal domestication have received less attention. Here, wepresent a nearly complete genome assembly for the recently described yeast species Saccharomyces eubayanus andcompare it to the genomes of multiple domesticated alloploid hybrids of S. eubayanus!S. cerevisiae (S. pastorianussyn. S. carlsbergensis), which are used to brew lager-style beers. We find that the S. eubayanus subgenomes of lagerbrewingyeasts have experienced increased rates of evolution since hybridization, and that certain genes involved inmetabolism may have been particularly affected. Interestingly, the S. eubayanus subgenome underwent an especiallystrong shift in selection regimes, consistent with more extensive domestication of the S. cerevisiae parent prior tohybridization. In contrast to recent proposals that lager-brewing yeasts were domesticated following a single hybridizationevent, the radically different neutral site divergences between the subgenomes of the two major lager yeast lineagesstrongly favor at least two independent origins for the S. cerevisiae!S. eubayanus hybrids that brew lager beers. Ourfindings demonstrate how this industrially important hybrid has been domesticated along similar evolutionary trajectorieson multiple occasions.