Local regulation of adrenal steroidogenesis: Is IL-1B involved?

SANTUCCI NATALIA; STAMPONE, ROCÍO; VILLAR, SILVINA; SPINELLI, SILVANA; BAY, MARÍA LUISA; BOTTASSO, OSCAR

Mar del Plata

Congreso; LXVI Reunión anual de la Sociedad Argentina de Inmunología; 2018

Adrenal steroidogenesis and immune responses (IR) are mutually regulated through the production of specific molecules helping to orchestrate an optimal response. Under pathological conditions, the IR attempts to eliminate the noxious stimulus and repair the damage whereas the endocrine response pursues to avoid an excessive inflammation. As an infectious disease, Tuberculosis is characterized by a substantial secretion of inflammatory cytokines like IL-1β, which is mainly produced by macrophages. While IL-1β modulates the adrenal steroidogenesis at the central level, it is not clear whether it exerts a local effect on the adrenal gland. Several studies suggest that Nuclear Receptors NR4As are potential key factors in inflammation and metabolism, for instance in steroidogenesis by modulating steroidogenic enzyme expression. Our former studies in the human cell line NCI-H295R (derived from a carcinoma of adrenal cortex), revealed that treatment with IL-1β 1.25 pg/ml + Fk (Forskolin), 24 h, increased cortisol and DHEA production respect cultures stimulated with Fk or left untreated. Cells exposed or not to Fk or IL-1β expressed type 1 and type 2 IL-1β receptors. Fk treatment increased IL-1R2 mRNA respect to IL-1R1 (p