INVESTIGADORES
MARTINEZ CERON Maria Camila
capítulos de libros
Título:
Peptide affinity chromatography based on combinatorial strategies for protein purification
Autor/es:
S. A. CAMPERI; CAMILA MARTINEZ CERON; S. L. GIUDICESSI; M. M. MARANI; F. ALBERICIO; O. CASCONE
Libro:
Protein Downstream Processing
Editorial:
Springer
Referencias:
Año: 2014; p. 277 - 302
Resumen:
We describe a method to develop affinity chromatography matrices with short peptide ligands for protein purification. The method entitles the following: a) synthesis of a combinatorial library on the HMBA-ChemMatrix resin by the divide-couple-recombine (DCR) method using the Fmoc chemistry, b) library screening with the protein of interest labeled with a fluorescent dye or biotin, c) identification of peptides contained on positive beads by tandem matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS), d) solid phase peptide ligands synthesis and immobilization in chromatographic supports, e) evaluation of protein adsorption on peptide affinity matrices from the equilibrium isotherms and breakthrough curves.
