INVESTIGADORES
MARTINEZ CERON Maria Camila
artículos
Título:
Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
Autor/es:
SOLEDAD L. SAAVEDRA; MARIA C MARTINEZ CERON; SILVANA L. GIUDICESSI; GUILLERMINA FORNO; MARÍA BOSCO; MARIELA M MARANI; ROSA ERRA-BALSELLS; FERNANDO ALBERICIO; OSVALDO CASCONE; SILVIA A. CAMPERI
Revista:
BIOTECHNOLOGY PROGRESS
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Washington; Año: 2018 vol. 34 p. 999 - 1005
ISSN:
8756-7938
Resumen:
Recombinant human growth hormone (rhGH) is used for the treatment ofseveral pathologies, most of them related to growth. Although differentexpression systems can be used for its production, the milk from transgeniccows is one of the most interesting due to the high rhGH level achieved (5 g/L).We have designed and synthesized short peptides (9 or 10 amino acid long)using Fmoc chemistry and studied its ability to purify rhGH from milk onceimmobilized on an agarose support. Using spiked milk with the hormone as asample, rhGH was purified with 88 % yield and 92 % purity in a single step witha fold purification of 4.5.
