INVESTIGADORES
ROLDAN OLARTE Eugenia Mariela
congresos y reuniones científicas
Título:
Identification of molecular genetic markers by means of RAPD in Vicuña genome
Autor/es:
LONGO, A.E; ROLDÁN-OLARTE, M; VALDECANTOS, P.A; GARCÍA, D.C; MICELI, D. C
Lugar:
Tafi del Valle, Tucumán
Reunión:
Jornada; XXIV Jornadas Científicas de la Asociación de Biología de Tucumán; 2008
Institución organizadora:
Asociación de Biología de Tucumán
Resumen:
The vicu (Vicugna vicugna) is a wild South American
Camelid. She was on the verge of extinction because of was
hunted for his fine fibre. Nowadays is not know the genetic
variability of vicuña populations of Argentina. In this work we
used RAPD molecular markers in order to study the genetic
variability of INTA Abra Pampa (Jujuy) vicuña population and
to identify news markers. The Random Amplified
Polymorphic DNA (RAPD) technique uses only primer of 10
nucleotides and his hybridization is to low temperature. Two
strategies were use for generating polymorphic patterns: 1)
RAPD with primers of 10 nucleotides and arbitrary sequence
(Series A, Biodynamics), 2) RAPD whit primers of 20
nucleotides whit 60-70% of C+G in the 3´ extreme (TGF βI,
TGF βII, BMP 15 and uPAR). The PCR reactions were
realized by genomic DNA obtained from blood and fibers to
a temperature of hibridization of 35ーC. The amplifications
products by possible polymorphism were selected and
isolated of the gel for his later purification, cloning and
sequence. The selected bands are moleculars markers
potentials and they can help to know more about the genetic
variability of vicu populations and her conservation.
Camelid. She was on the verge of extinction because of was
hunted for his fine fibre. Nowadays is not know the genetic
variability of vicuña populations of Argentina. In this work we
used RAPD molecular markers in order to study the genetic
variability of INTA Abra Pampa (Jujuy) vicuña population and
to identify news markers. The Random Amplified
Polymorphic DNA (RAPD) technique uses only primer of 10
nucleotides and his hybridization is to low temperature. Two
strategies were use for generating polymorphic patterns: 1)
RAPD with primers of 10 nucleotides and arbitrary sequence
(Series A, Biodynamics), 2) RAPD whit primers of 20
nucleotides whit 60-70% of C+G in the 3´ extreme (TGF βI,
TGF βII, BMP 15 and uPAR). The PCR reactions were
realized by genomic DNA obtained from blood and fibers to
a temperature of hibridization of 35ーC. The amplifications
products by possible polymorphism were selected and
isolated of the gel for his later purification, cloning and
sequence. The selected bands are moleculars markers
potentials and they can help to know more about the genetic
variability of vicu populations and her conservation.
Camelid. She was on the verge of extinction because of was
hunted for his fine fibre. Nowadays is not know the genetic
variability of vicuña populations of Argentina. In this work we
used RAPD molecular markers in order to study the genetic
variability of INTA Abra Pampa (Jujuy) vicuña population and
to identify news markers. The Random Amplified
Polymorphic DNA (RAPD) technique uses only primer of 10
nucleotides and his hybridization is to low temperature. Two
strategies were use for generating polymorphic patterns: 1)
RAPD with primers of 10 nucleotides and arbitrary sequence
(Series A, Biodynamics), 2) RAPD whit primers of 20
nucleotides whit 60-70% of C+G in the 3´ extreme (TGF βI,
TGF βII, BMP 15 and uPAR). The PCR reactions were
realized by genomic DNA obtained from blood and fibers to
a temperature of hibridization of 35ーC. The amplifications
products by possible polymorphism were selected and
isolated of the gel for his later purification, cloning and
sequence. The selected bands are moleculars markers
potentials and they can help to know more about the genetic
variability of vicu populations and her conservation.
Vicugna vicugna) is a wild South American
Camelid. She was on the verge of extinction because of was
hunted for his fine fibre. Nowadays is not know the genetic
variability of vicuña populations of Argentina. In this work we
used RAPD molecular markers in order to study the genetic
variability of INTA Abra Pampa (Jujuy) vicuña population and
to identify news markers. The Random Amplified
Polymorphic DNA (RAPD) technique uses only primer of 10
nucleotides and his hybridization is to low temperature. Two
strategies were use for generating polymorphic patterns: 1)
RAPD with primers of 10 nucleotides and arbitrary sequence
(Series A, Biodynamics), 2) RAPD whit primers of 20
nucleotides whit 60-70% of C+G in the 3´ extreme (TGF βI,
TGF βII, BMP 15 and uPAR). The PCR reactions were
realized by genomic DNA obtained from blood and fibers to
a temperature of hibridization of 35ーC. The amplifications
products by possible polymorphism were selected and
isolated of the gel for his later purification, cloning and
sequence. The selected bands are moleculars markers
potentials and they can help to know more about the genetic
variability of vicu populations and her conservation.