In vivo interaction between P-bodies components in Drosophila melanogaster.

CORUJO GH; FERRERO PV; RIVERA POMAR R; LAYANA C

Rosario

Congreso; L Reunión Anual de la Sociedad argentina de Investigación Bioquímica y Biología Molecular; 2014

SAIB

Gene expression is a highly regulated process. Translation regulation controls of cellular proteome, enabling prompt quantitative and qualitative changes in response to the environment. Cytoplasmic processing bodies (PB) are discrete cytoplasmic foci involved in mRNA silencing, surveillance and degradation (Andrei et al., 2005; Cougot et al., 2004; Eystathioy, 2003; Layana et al., 2012; Sheth and Parker, 2003). We study the dynamic of mRNP remodeling in PB and the mechanisms that promote the silencing of mRNA active in polysomes. RNAi assays demonstrated that the presence of some factors, in particular eIF4E is crucial to PB formation (Andrei et al., 2005; Eulalio et al., 2007). We have showed that different isoforms and eIF4E mutants are required for PB localization (Ferrero et al., 2012) Here we analyzed in detail the interactions between some components of PB, namely eIF4E-1, eIF4E-3, Lsm-1, y Me31B in Drosophila melanogaster. In vitro interaction was demonstrated by two hybrid assays, and in vivo analysis of interactions in PB in Drosophila S2 cells was studied by FRET. Our results demonstrated in S2 cells that the isoforms of the cap binding protein eIF4E-1 and eIF4E-3 interact with Me31B and Lsm-1 and that this interaction has implicances in the control of mRNA silencing in somatic cells and in the germ line of Drosophila melanogaster.