Is NMMIIA part of the transcription/splicing machinery?

LAYANA C; DIAZ VM; RIVERA POMAR R; NAUM ONGANIA G

Heidelberg

Simposio; Lessons for Integrating Structure, Biophysics and Chemistry; 2014

European Molecular Biology Organization

The DNA-binding dimer Pknox1-Pbx1 modulates Hox protein activity. Non muscle myosin II A (NMMIIA) is a cytoplasmic protein that works in concert with polymeric actin to produce cell movement. It is involved in a variety of processes including muscle contraction, cell locomotion, cell division and receptor capping. In previous work, it has been found that Pknox1-Pbx1 interacts with a transcription/splicing factor. Moreover, we have purified NMMIIA with Pknox1 together with the nuclear speckle factor THOC, transcription/splicing factors PSF and p54(nrb), the elongating RNA Pol II and the actin polymerization inducer, N-WASP. Here, we found that NMMIIA is present in the nucleus of mammalian cells. Moreover, NMMIIA interacts with Pbx1, suggesting that it could have a mechanical role in the transcription/splicing process of the Hox genes.