ABNORMAL/DYSFUNCTIONAL DENDRITIC CELL GENERATION FROM BONE MARROW OF LIPOPOLYSACCHARIDE-IMMUNOSUPPRESSED MICE

VERÓNICA I. LANDONI; NAHUEL RODRIGUEZ-RODRIGUES; PAULA CHIARELLA; DAIANA MARTIRE GRECO,; LUIS CASTILLO; PABLO SCHIERLOH,; ISTURIZ MARTIN A.;; GABRIELA C. FERNÁNDEZ.

Congreso; LXIV Reunión Anual De La Sociedad Argentina De Inmunología (Sai); 2016

An immunosuppressed state is elicited in some patient at latephases of sepsis, evidenced by a reduced T cell response andantibody production. A loss of dendritic cells (DC) in blood andlymph nodes (LN) has been also described in septic patients.Considering this, we hypothesized that alterations in the number/function of DC may contribute to the immunosuppression (IS)associated to sepsis. Repetitive, increasing doses of LPS in micemimics this state of IS. The aim of this work was to evaluate thephenotype and functional state of DC from IS mice. We found thatthe number of DC (CD11c+) in LN of IS mice was lower compareto Control (Ctrol). Then we traced DC migration to LN by theFITC skin painting method. We found a reduced number of DC(CD11c+FITC+) mobilized to LN in IS mice (p