MYELOID IMMATURE CELLS (GR-1+CD11B+) ACQUIRE THEIR INHIBITORY PROPERTIES IN THE BONE MARROW, AND MIGRATE TO LYMPH NODES TO EXERT THEIR FUNCTION

VERÓNICA I. LANDONI; DAIANA MARTIRE GRECO,; NAHUEL RODRIGUEZ-RODRIGUES; CHIARELLA PAULLA; PABLO SCHIERLOH,; BÁRBARA REARTE; MARTÍN A. ISTURIZ; GABRIELA FERNANDEZ

Congreso; LIX REUNIÓN CIENTÍFICA ANUAL-LXII REUNIÓN ANUAL; 2014

Lymph nodes (LN) from LPS-immunosuppressed (IS) mice have a decreased T cell (Ly) proliferation, associated to an increment of myeloid derived suppressor cells (MDSC, Gr-1+CD11b+). Moreover, MDSC from bone marrow (BM) of IS mice also exhibited an inhibitory activity towards Ly proliferation. Our aim was to evaluate whether MDSC acquire their immunosuppressive properties in BM and then migrate to LN to exert their function, and evaluate their inhibitory mechanisms. We found that the inhibition of Ly proliferation by BM-IS was accompanied with an increase in cells expressing inducible nitric oxide (NO) synthase (Gr-1+CD11b+iNOS+) and reactive oxygen species (ROS) (Gr-1+CD11b+DHR+) determined by FACS (p