Influence of the polymer Morphology of sorbents on their properties in affinity chromatography

CECILIA I. ALVAREZ IGARZABAL; CESAR G. GOMEZ; MIRIAM C. STRUMIA

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2001 vol. 49 p. 141 - 151

0165-022X

The influence of the polymeric morphology of different types of Fe3 q-containing sorbents and their properties in retention of phosphoamino acids is presented in this paper. Poly hydroxylated? . w polybutadienic-hydroxyethyl methacrylate poly PB-HEMA and poly ethylene glycol? .x ? dimethacrylate-hydroxyethyl methacrylate poly EGDMA-HEMA base supports were submitted. w ? .x to chemical modifications to attain metal ion-containing sorbents. Properties such as specific surface area, pore volume, equilibrium volume swelling ratios, extent of conversion rate of functional groups, amount of chelated metal ion, ligand occupation, as well as quantity of phosphoamino acid retained, were used as comparative parameters for those different base matrices. Results suggest that Fe immobilized on poly EGDMA-HEMA base support are more3q ? . efficient as a group-specific sorbent to retain phosphoamino acids than those obtained using poly PB-HEMA base support.