Surface modification on poly (EGDMA-co-HEMA) synthetic matrices to be used as specific adsorbents

CESAR G. GOMEZ; CECILIA I. ALVAREZ IGARZABAL; MIRIAM C. STRUMIA

POLYMER

Elsevier

Año: 2005 vol. 46 p. 6300 - 6307

0032-3861

The preparation and modification of ethylene glycol dimethacrylate/2-hydroxyethyl methacrylate copolymers [poly (EGDMA-co- HEMA)] were carried out to study their properties on Immobilized Metal Ion Affinity Chromatography (IMAC). For that purpose, the adsorption–desorption assays of a phosphoprotein, ovalbumin (OVA) on different ion-containing adsorbents were investigated. The metal ion-complexing agent used was iminodiacetic acid (IDA) and the metal ions chelated were Al3C or Fe3C. The efficiency on both modification reactions and OVA retention was analysed taking into account the pore volumes (Vp) and specific surface area (Ss) values in macropores zones and equilibrium volume-swelling ratio (qv) from base matrices and final ion-containing supports. In general, Fe3Ccontaining products resulted more efficient adsorbents in the OVA retention and although the adsorption assays on Fe3C or Al3C-containing supports were fast, the complete and faster OVA desorption was reached when Al3C-containing matrix was used.co- HEMA)] were carried out to study their properties on Immobilized Metal Ion Affinity Chromatography (IMAC). For that purpose, the adsorption–desorption assays of a phosphoprotein, ovalbumin (OVA) on different ion-containing adsorbents were investigated. The metal ion-complexing agent used was iminodiacetic acid (IDA) and the metal ions chelated were Al3C or Fe3C. The efficiency on both modification reactions and OVA retention was analysed taking into account the pore volumes (Vp) and specific surface area (Ss) values in macropores zones and equilibrium volume-swelling ratio (qv) from base matrices and final ion-containing supports. In general, Fe3Ccontaining products resulted more efficient adsorbents in the OVA retention and although the adsorption assays on Fe3C or Al3C-containing supports were fast, the complete and faster OVA desorption was reached when Al3C-containing matrix was used.3C or Fe3C. The efficiency on both modification reactions and OVA retention was analysed taking into account the pore volumes (Vp) and specific surface area (Ss) values in macropores zones and equilibrium volume-swelling ratio (qv) from base matrices and final ion-containing supports. In general, Fe3Ccontaining products resulted more efficient adsorbents in the OVA retention and although the adsorption assays on Fe3C or Al3C-containing supports were fast, the complete and faster OVA desorption was reached when Al3C-containing matrix was used.Vp) and specific surface area (Ss) values in macropores zones and equilibrium volume-swelling ratio (qv) from base matrices and final ion-containing supports. In general, Fe3Ccontaining products resulted more efficient adsorbents in the OVA retention and although the adsorption assays on Fe3C or Al3C-containing supports were fast, the complete and faster OVA desorption was reached when Al3C-containing matrix was used.qv) from base matrices and final ion-containing supports. In general, Fe3Ccontaining products resulted more efficient adsorbents in the OVA retention and although the adsorption assays on Fe3C or Al3C-containing supports were fast, the complete and faster OVA desorption was reached when Al3C-containing matrix was used.3C or Al3C-containing supports were fast, the complete and faster OVA desorption was reached when Al3C-containing matrix was used.3C-containing matrix was used.