Analysis of pelvic pain development, mast cell infiltration and prostate inflammation in an animal model of Chronic Pelvic Pain Syndrome

SALAZAR F; BRESER ML; GODOY GJ; RIVERO VE; MOTRICH RD

Buenos Aires

Congreso; Reunión conjunta de sociedades de biociencias- LXII Reunión anual de la Sociedad argentina de investigación clínica (SAIC)- LIII Reunión anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB)- LXV Reunión anual de la Socied; 2017

Reunión conjunta de las 10 sociedades de biociencias de la Argentina

Pain and inflammation in the absence of infection are hallmarksin Chronic Pelvic Pain Syndrome (CPPS) patients. In this set, mastcells have been pointed out as key players in pain induction andcentral sensitization. Although its etiology remains unclear, autoimmunityhas been proposed as a cause and animal models of ExperimentalAutoimmune Prostatitis (EAP) models have long been usedfor studying CP/CPPS.Herein, we analyzed prostate inflammation induction, mast cellinfiltration and chronic pelvic pain development in EAP in threecommon laboratory mouse strains presenting with differential susceptibilityto autoimmune prostatitis: the NOD mice (highly susceptible),the C57BL/6 mice (moderately susceptible), and the BALB/cmice(resistant).Chronic pelvic pain development, evidenced by increased tactileallodynia responses, was similar between immunized NOD andC57BL/6 mice, although the severity of leukocyte infiltration andinflammation was greater in the first case. In fact, prostate tissuefrom NOD mice revealed markedly increased expression levels ofinflammatory cytokines and chemokines. Similar results, but to alesser extent, were observed when analyzing prostate tissue fromC57BL/6 mice. However, similarly increased numbers of mast cells,mostly degranulated, were detected in prostate samples from eitherNOD or C57BL/6 mice. Conversely, minimal prostate leukocyte infiltrationand inflammation was observed in immunized BALB/c mice.Besides, they showed no pelvic pain development and the lowestprostate tissue mast cell total counts and in a resting state.Our results provide new evidence indicating that NOD, C57BL/6,and BALB/c mice develop different degrees of chronic pelvic pain,type and amount of prostate inflammation and mast cell infiltration.Remarkably, chronic pelvic pain development correlated with mastcell infiltration, suggesting that mast cells are inflammatory cells involvedin mediating pelvic pain induction.