Study of specific T cell subsets and immunodominant antigens involved in the development of experimental autoimmune prostatiti

MORICH RD; DANESHJOU N; BRESER ML; SANCHEZ LR; BRACIAK T; SERCARZ E; RIVERO VE

Los Cocos

Congreso; LXI Reunión Científica de la Sociedad Argentina de Inmunología; 2013

Sociedad Argentina de Inmunologia

The Experimental Autoimmune Prostatitis (EAP) model developed in NOD mice is valid for the study of Chronic Non Bacterial Prostatitis. Herein, we aimed to advance in the current understanding of the immune mechanism involved by the analysis of specific T cell populations and their repertoire. NOD mice 6-8 week old were immunized with a prostate extract of proteins (Group EP), a purified antigen called Prostatein (Group PSBP), PSBP peptides (Groups P1 and P2), or saline solution (Group C) on days 0 and 15. Animals were euthanized on days 10 and 24, and the immune response and secreted cytokines were analyzed. Adoptive transfer of CD3+ cells from immunized animals, or of short term prostate-specific T cell lines to NOD-SCID recipients were performed and the TCR repertoire of prostate infiltrating lymphocytes was analyzed. Autoimmune animals (Groups EP and PSBP) developed specific T cell responses to PSBP with secretion of high levels of IFNg, IL17 and IL2, and almost null levels of IL4 (p