Rol of INFγ, IL17 and IL10 producing cells in an experimental model of autoimmune prostatitis

BRESER ML; BERTOTTO ME; MACKERN-OBERTI JP; SANCHEZ L; MACCIONI M; RIVERO VE

Buenos Aires

Congreso; First French-Argentine Immunology Congress (FAIC), LVIII Reunion Anual de la Sociedad Argentina de Inmunología.; 2010

Sociedad Argentina de Inmunologia y Sociedad Francesa de Inmunologia

Extensive work has been done regarding the development and characterization of rodent models of experimental autoimmune prostatitis (EAP), models that could be considered as appropriate animal models for the human disease named Chronic prostatitis/chronic pelvic pain syndrome. In the present work we analyze the role of INFγ, IL10 and IL17 producing cells in autoimmune prostatitis. Prostate extract plus adjuvant was used to immunize Balb/c, NOD and NOD INFγ deficient (NODINFγ-/-) mice. Control groups, immunized just with adjuvant were also included. Mice were sacrificed at day 24 after immunization and the presence of antigen specific INFγ, IL10 and IL17 producing cells was evaluated by ELISA and FACS. Infiltration of the prostate gland was also analyzed by conventional histology and FACS. No antigen specific INFγ, IL10 and IL17 producing cells were observed in lymph nodes and spleen of control mice of the 3 strains tested. A high secretion of specific IL17 was observed in culture supernatants of Balb/c, NOD and NODINFγ-/- mice (Balb/c: 356±53 pg/ml; NOD: 870±94 pg/ml; NODINFγ-/-: 1678±233 pg/ml). Specific IL10 secretion was mainly observed in culture supernatants of Balb/c and NODINFγ-/- mice (Balb/c: 230±20 pg/ml; NOD: 89±16 pg/ml; NODINFγ-/-: 150±40 pg/ml). A significant elevated secretion of specific INFγ was observed in culture supernatants of NOD mice (Balb/c: 370±25 pg/ml; NOD: 4039±660 pg/ml; NODINFγ-/-: ND). When histological infiltration was analyzed higher severity scores were observed in NOD prostate glands while minor infiltration was detected in Balb/c and NODINFγ-/-. Prostate gland infiltration observed in NOD mice was composed mostly by CD3 cells with high proportion of CD8 and CD4 cells. These results indicate that the presence of specific INFγ producing cells is highly associated with marked infiltration of the prostate gland, while the only presence of IL-17 producing cells is not enough to generate the disease.