INVESTIGADORES
EDREIRA Martin Miguel
congresos y reuniones científicas
Título:
A NOVEL Trypanosoma cruzi cAMP EFFECTOR PROTEIN
Autor/es:
JÉSICA MILD, DANIEL MUSIKANT, GABRIEL FERRI, BÁRBARA MC CORMACK, MARTÍN M EDREIRA
Reunión:
Congreso; X Congreso de Protozoología y enfermedades PArasitarias; 2014
Institución organizadora:
Sociedad Argentina de Protozoologia
Resumen:
Although cAMP plays an essential role in Trypanosoma cruzi life cycle, the exact
mechanisms of action are unknown. Up to date, Protein Kinase A (PKA) is the only known
T. cruzi cAMP effector, however experimental evidences suggest the existence of other
effectors. In order to identify new cAMP effectors, we carried out in silico studies using the
predicted T. cruzi proteome. As a result, we identified 27 proteins with putative cAMP
binding domains. Within these proteins, we subcloned and characterized the paralogs
proteins TcCLB.508523.80 and TcCLB.507035.110, the two most solid candidates.
Computational models shown that the domain present in these proteins could fold in a
similar manner than other know cAMP effectors. In fact, both proteins could specifically
bind cAMP. Moreover, transgenic T. cruzi epimastigotes expressing TcCLB.508523.80
shown an increased infectivity when incubated with 8-Br-cAMP. Our results indicate that
TcCLB.508523.80 and TcCLB.507035.110 are bona fide cAMP binding proteins that could
act as novel T. cruzi cAMP effectors during host cell infection