Labeling of the anti-melanoma 14f7 monoclonal antibody with rhenium-188-MAG(3) chelate: Conjugation optimization, in vitro stability and animal studies

CRUDO JL, EDREIRA MM, OBENAUS ER, DE CASTIGLIA SG

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY

Año: 2004 vol. 261 p. 337 - 342

0236-5731

Rhenium-188 is a high energy -emitter that has a therapeutic promise. The aim of the present study was to label the anti-melanoma 14f7monoclonal antibody (MoAb) with 188Re, using an active ester of S-benzoyl-mercaptoacetyltriglycine (S-benzoyl-MAG3) and to evaluate its tumoruptake in C57 mice bearing a B16F0 melanoma. The optimum labeling efficiency was 49.6% and the maximal tumor uptake was 8.8%ID/g at 24hours. Quality assurance tests were performed during the entire labeling procedure and on the final radiolabeled MoAb. Stability studies in salineand human serum were also performed.