NEONATAL EXPOSURE TO A GLYPHOSATE-BASED HERBICIDE ALTERS UTERINE ADENOGENESIS IN EWE LAMB.

ALARCÓN R.; MILESI M.; RIVERA, OSCAR; INGARAMO P. I.; MUÑÓZ DE TORO M; LUQUE EH

Santa Fe

Congreso; Reunion conjunta de sociedades de biociencias; 2017

SAIC-SAIB-SAI-SAA-SAB-SAFE-SAFIS-SAH-SAP

Abstract: Glyphosate based herbicides (GBH) are one of the mostextensively used pesticides in agriculture all over the world. Recently,we showed that a brief exposure to a GBH during the first weekof life alters uterine development and induces epithelial hyperplasiain prepubertal rats, and causes post-implantation embryo loss atadulthood. This study investigates the effects of a brief postnatalexposure to a low dose of GBH on the differentiation of the prepubertalewe lamb uterus. Ewe lambs (Frizone breed) were sc injectedfrom postnatal day 1 (PND1) to PND14 with saline solution (vehicle)or 2 mg/Kg/day of a GBH (reference dose from EPA, USA). OnPND45 ewe lambs were hysterectomized and uterine samples wereparaffin-embedded or stored at -80°C until mRNA extraction. Cellproliferation was assessed in all uterine compartments by quantifyingthe expression of Ki-67 protein through immunohistochemistry(IHQ). We also determine the uterine expression of Forkhead boxA2 (Foxa2) protein by IHQ, which is involved in the uterine glanddevelopment. RT-PCR was performed to evaluate the expression ofIGF family genes related with the cell proliferation, i.e., insulin-likegrowth factor-1 (IGF-1), IGF-2 and its receptor (IGF-1R). GBH treatmentdecreased the proliferation rate in the subepithelial stroma(7.7±0.9% vs C:12.2±1.5%, p