SALVIA HISPANICA L. (CHIA) SEED IMPROVES LIVER INFLAMMATION IN AN EXPERIMENTAL MODEL OF METABOLIC SYNDROME

VEGA JOUBERT, MICHELLE BERENICE; INGARAMO, PAOLA; OLIVA M.E.; D'ALESSANDRO M.E.

Congreso; Reunión Conjunta SAIC SAI SAFIS Noviembre 2022; 2022

Non-alcoholic fatty liver disease (NAFLD) is recognized as the liverdisease component of Metabolic Syndrome (MS). Inflammationclearly play a pivotal role in the progression of this disease process.Objectives: The aim of the present study was to analyze liver inflammationin an experimental model of MS induced by chronic administrationof a sucrose-rich diet (SRD) and to evaluate the effectsof chia seed as therapeutic strategy. Materials and methods: MaleWistar rats were fed with a reference diet (RD) -6 months- or a SRD-3 months-. Then, the latter group was randomly divided into twosubgroups. One subgroup continued receiving the SRD for up to 6months and the other was fed with a SRD where whole chia seedwas incorporated as a source of dietary fat for the next 3 months(SRD+CHIA). We analized: in serum: triglycerides, cholesterol, glucoseand insulin, interleukin-6 (IL-6) and TNFα. In liver: NAS (NAFLDactivity score), IL-1β, IL-10, nuclear factor-kB (NFkB), PAI-1,F4-80 expression, MPO activity and Nuclear erythroid 2-related factor2 (NrF2). Results: The results showed that rats fed with a SRDdeveloped dyslipidemia, hyperglycemia and inflammation. HepaticNAS, IL-1β, NFκB p65, PAI-1, F4-80 expression, MPO activity weresignificantly increased and IL-10 expression was decreased. Thiswas acompanied for an increased plasma IL-6 and TNF-α levels. Inaddition, we observed a significant decrease in liver Nrf2. The administrationof chia seed for the last 3 months reversed dyslipidemia,hyperglycemia and inflammation. In the liver: NAS, IL-1β, IL-10,PAI-1, F4-80, NFκB, NrF2p65 and MPO activity were normalized.Conclusion: This study showed new aspects on liver inflammation indyslipidemic insulin resistant rats chronically fed with a sucrose-richdiet. In addition, we demonstrated new properties and molecularmechanisms associated with beneficial effects on inflammation ofchia seed as a therapeutic strategy.