Study Of Toxicity And Cellular Uptake Of Magnetic Nanoparticles With Different Coatings

SELZER SOLANGE M.,; BONNET LAURA V.; FERREYRA NANCY F.; VICO RAQUEL

Venecia

Congreso; GREEN CHEMISTRY POSTGRADUATE SUMMER SCHOOL; 2021

Green Sciences for Sustainable Development Fundation

Currently, magnetite nanoparticles (MNPs) are widely studied for biotechnological applications due their low toxicity and the simplicity and low cost of its synthesis. Their high ratio surface/volume may be exploit for incorporate in their surface different kind of molecules, that give them colloidal stability and specificity for biorecognition or biosensing [1]. A great number of cellular process and diseases have biomarkers that involve the interaction between carbohydrates and proteins. Because of this, biosensing is subject of large studies and nanoparticles results an interesting option to be used as plataforms [2]. In this context, biological environment effects on nanoparticles identity should be well established in order to ensure a well sensing. However, there is still lack of the knowledge about interactions between MNPs coated with different molecules and cells, as well as the repercussion of these on biological systems. In order to carry out the functionalization of nanoparticles with biomolecules (carbohydrates or proteins) that will act as recognition element, an accessible surface chemistry is necessary. Thus, stabilized nanoparticles should be the key for the biomolecules binding to the surface. In this work, cytotoxicity and cellular uptake of unstabilized nanoparticles (MNPs) and stabilized with different functional groups, amino (MNP-Ar-NH2) and carboxylic acid (MNP-Ar-COOH) has been studied. None of the nanoparticles have been toxic to CHO K1 hamster ovary cells and have shown differential cellular uptake in Hella LC3 gfp cells. Hella LC3-gfp cells have a green fluorescent gene that shows the degradation pathway called autophagy. These results shown that MNP-Ar-NH2 were more internalized compared to MNPs and MNP-Ar-COOH. This fact may be due to the type of proteins that form the crown around the surface, which will have a composition that will depend on the surface charge, coating degree, among other factors.