Development and characterization of nano-vehicles based on argentinian Chitosan:promising carriers for the encapsulation and protection of Resveratrol

BUOSI FB; CASTAÑEDACATAÑA MA; MARTÍNEZ KD; DISANTO MC; DOMINGUEZ RUBIO AP; ALAIMO A; PÉREZ OE

Polo Científico y Tecnológico. Capital Federal

Simposio; Simposio Fronteras en Biociencias, Polo Científico y Tecnológico; 2018

IBioBA, CONICET - Partner Institute of the Max Planck Society

Resveratrol (RSV) is a natural polyphenol which isrich in a variety of plants and fruits, such as grapes, peanut and mulberry.This compound has been shown of possessing multiple pharmacological and healthybenefits, including anti-oxidation/inflammation. However, the biomedicalapplications of RSV remain limited due to its short biological half-life,labile properties, and rapid metabolism and elimination.Chitosan (Chi) is a naturally occurring and abundantlyavailable polysaccharide extensively studied for drug delivery systems due tothe biological properties such as its relative non toxicity, biocompatibility,biodegradability, cationic properties and mucoadhesive characteristics. Chi andtripolyphosphate-sodium (TPP, cross-linking agent) can form biocompatible,cross-linked spheres through electrostatic interactions that can be efficientlyemployed in bioactives delivery. On the other hand, treatment of diseases of the posteriorsegment of the eye, such as Age-related Macular Degeneration (AMD), requiresnovel drug delivery systems that can overcome the many barriers for efficaciousdelivery of therapeutic drugs. This challenge has prompted us to develop aninnovative Chi-TPP nanosystem for the encapsulation, protection and controlledrelease of RSV and its subsequent evaluation in an in vitro model of AMD. Results: colloidal particles of Chi-TPP(3mg/ml-0.25mg/ml) were obtained by ionic gelation. The particle sizedistribution and the ζpotentialwere recorded by dynamic light scattering (DLS, Malvern Z equipment). The peaksof the majority populations were located at 50 and 500 nm. A decrease in themaximum fluorescence peak of RSV was quantified by interaction with Chi. Inaddition, the protective effect of Chi against UV (254nm, 15W, 90 min)-inducedphotodegradation of RSV was determined by spectrophotometry (λem = 306nm; POLARstarOmega).Based on our findings, we envision that the nanoencapsulationwith Chi-TPP nanospheres could be potential carrier for RSV, opening thepossibility of its application for ophthalmic use.